Most of the aches and pains have subsided, allergy medication has been obtained, and life has returned to normal. I got my bike fixed, so now I also have no excuse for being out of shape.
It looks like Jorge's going to let me spend $1000 on getting a gene synthesized from scratch. I've been trying to overexpress and purify this Geobacillus enzyme in E. coli, and the codon usage is so different from what coli uses that I'm barely getting any product. So, rather than struggle with it any more, we send money to this company, they figure out what codons would work best in coli, whip it up in their DNA synthesizer, and voila: gene. If it works, it'll be great! (Another exciting adventure in science!)
No comments:
Post a Comment