Glorious victory! Finally figured out how to knock out genes in Rhodobacter sphaeroides! Only took 3 years, but I've got the little punks now. Turns out the secret is that the excission event only happens in about 1 in every 10^11 cells. Do the counter selection with a 100 ml inoculum, and you're golden.
I hope it has an interesting phenotype, after all this time. :-)
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